flowAI report

Quality control analysis

Summary

The anomalies were removed from: Flow Rate, Flow Signal and Flow Margin
Anomalies detected in total: 58.46 %
Number of high quality events: 731740

Flow rate check

13.22 % anomalies detected in the flow rate check.

The plot reconstructs the flow rate with a resolution of 1/10 of a second. Anomalies in the flow rate are identified with an algorithm based on the generalied ESD outlier detection method. The anomalies are circled in green.

Signals acquisition check

55.21 % anomalies detected in the signal aquisition check.

The more stable region selected consistent for all channels is highlighted in yellow. If the removal of outliers has been required before the execution of the changepoint analysis, the detected outliers are cicled in green.

The FCS file was divided in 3523 bins, hence each bin contains 500 event(s). The stable region is located between the bins 699 and 2276.

More info on the changepoints detected

Changepoints detected in channels with shifts in signal acquisition:

##                 1    2    3
## 488 525/50-A 2646 3229     
## 488 715/30-A 2544 3085 3416
## 405 450/50-A 3081          
## 405 515/20-A 3081 3367     
## 405 605/40-A 3082          
## 405 670/30-A 3023          
## 405 710/40-A 2988          
## 355 379/28-A 2579          
## 355 515/30-A  699 2577 3270
## 355 560/40-A  699 2585 3197
## 355 740/35-A 2276 3453     
## 640 670/30-A 3351          
## 561 585/15-A 2599 3399     
## 561 610/20-A 2599 3399     
## 561 780/60-A 2571          
## 405 585/15-A 3079

Channels excluded from the analysis: FSC-A, FSC-W, SSC-A, SSC-W.

No changepoints were detected in the channels: 405 780/60-A, 355 820/60-A, 640 730/35-A, 640 780/60-A.

Dynamic range check

0.86 % anomalies detected in dynamic range check.

The plot shows where the anomalies occured the most. The x-axis scale is complementary to the one of the signal acquisition plot.

More info on the anomalies detected in the dynamic range check

For each channel analyzed, the table shows the number of events that did not pass the dynamic range check:

##              lower_range upper_range
## 488 525/50-A         735        2829
## 488 715/30-A         194          61
## 405 450/50-A         398         655
## 405 515/20-A         313        3598
## 405 605/40-A         230         166
## 405 670/30-A         245         101
## 405 710/40-A         213          57
## 405 780/60-A         539           7
## 355 379/28-A         424         403
## 355 515/30-A         422        1781
## 355 560/40-A         330         247
## 355 740/35-A         106          13
## 355 820/60-A         810           5
## 640 670/30-A         126          23
## 640 730/35-A         120          15
## 640 780/60-A         264          21
## 561 585/15-A         179        9107
## 561 610/20-A         111        4487
## 561 780/60-A         197          25
## 405 585/15-A         224         248
## total_SUM           6180       23849
## total_UNIQUE        1732       13352